100213820 RECOMBINATION DNA TECHNOLOGY & GENETIC ENGINEERING ( 3 Crd.Hrs )

Pre-Requisites : 100213311

The laboratory experiments illustrate the maximum of the following current concepts in the recombinant DNA technology and genetic engineering: DNA extraction from plant and animal tissues, evaluation of the concentration of DNA, preparation of competent cells mini -preparation of plasmid DNA, DNA digestion (restriction enzymes), restriction mapping of DNA, cloning of DNA, bacterial transformation, PCR, electro-elution of DNA from agarose RAPD (Random Amplified Polymorphic DNA)