Based on an old literature, field surveys and traditional medicine we have already identified plants for the treatment of cancer disease. However, the effect of medicinal plants on tumour progression awaits further studies. Even though, there is accumulating evidence that strongly suggests the implication of medicinal plants in the treatment of cancer. Therefore, this project will concentrate on studying the plant extracts that might be implicated in apoptosis induction. This is of a major significance in such a multifactorial malignancy, since apoptosis can be the goal of both preventive and therapeutic interventions. The first phase of studying the efficacy of chemicals is cell lines. Hence, water-ethanol (50%, 50%) extracts of Eryngium creticum (leaf) and Ziziphus spina-christi (leaf and fruit) will be applied on human prostate cancer cells (PC3), skin cancer cells (B16) and lung cancer cells (A547). Ethidium Bromide/Acridine Orange staining method will be employed to detect apoptotic cells. MTT assay will be used to determine the non -toxic concentration of the plants extract.
This study aims to (1) screen the effect of diverse potential therapeutic plants extracts on apoptosis induction in several cancer cell lines including human prostate, skin cells and lung cancer cells. We will focus on sensitization of cancer cells to apoptosis induction as a promising strategy in cancer therapy. (2) Test the toxicity of the tested plants extracts on normal (non carcinogenic) cells, i.e. skeletal muscle cells.
The finding of new healthcare concepts takes considerable time, and is not predictable. However it does require an initial investment in research, collection and protection of know-how, which must be done before the knowledge disappears or becomes unobtainable. Revealing the action mechanism of Eryngium creticum and Ziziphus spina-christi, two plants that grow in Palestine, is economically and academically beneficial.