Optimization of a Cultural Medium for Antifungal Antibiotic Production by Strain no.10/2(S. albovinaceus)

A broad antifungal and antibacterial activity of a new actinomycete (S. albovinaceus) strain no.10/2 was isolated from soil sample of Palestine and characterized. The medium composition for antifungal production for strain no.10/2 (S. albovinaceus) was optimized using shake-flask methodology. 1.25 % mannitol and 1 % malt extract were found to be best carbon and nitrogen sources respectively for growth and antifungal production. Similarly initial pH of 7.2, 10 % level of inoculum, incubation period of 96 h, and agitation in 250 ml bottom indented flask and medium to flask ratio of 1:10 (aeration) were found to be optimal. Optimization of medium and cultural conditions resulted in good increase in antifungal yield.