fbpx Oxygen tension, insulin, and glucagon affect the preservation and induction of cytochrome P450 isoforms in cultured rat hepatocytes |ARAB AMERICAN UNIVERSITY
Contact information for Technical Support and Student Assistance ... Click here

Oxygen tension, insulin, and glucagon affect the preservation and induction of cytochrome P450 isoforms in cultured rat hepatocytes

Authors: 
Bashar Saad, Helmut Thomas, Hanspeter Schawalder, Felix Waechter, Peter Maier
Journal Name: 
Toxicology and applied pharmacology
Volume: 
126
Issue: 
2
Pages From: 
372
To: 
379
Date: 
Wednesday, June 1, 1994
Abstract: 
The role of oxygen tension, insulin, and glucagon on the preservation and induction of cytochrome P450 isoenzyme activities and contents was investigated in rat hepatocytes cultured for 4 days on crude liver membrane fractions at 4 or 13% O2. At 13% O2, three out of six immunochemically analyzed P450 isoenzymes were significantly higher than in 4% O2. Exposure to phenobarbital (PB) from Days 1 to 4 dose dependently increased the protein content and decreased the albumin secretion in 13% O2 cultures only. The ...