Journal Name:
Electrochimica Acta
Volume:
48
Issue:
20
Pages From:
3349
To:
3354
Date:
Tuesday, September 9, 2003
Keywords:
Nitric oxide; Manganese porphyrin; Human umbilicalvein endothelial cells; Endothelial cell; Conducting polymer; Microsensor;Modified electrode
Abstract:
Pt-disk electrodes (50 μm diameter) were modified with pyrrole-functionalised Mn(II) trimethoxycarboxyphenylporphyrin by means of an electrochemical pulse technique leading to stable and sensitive microsensors that displayed electrocatalytic activitiy suitable for the detection and quantification of nitric oxide (NO). In order to improve selectivity, a thin layer of Nafion® was deposited on top of the porphyrin-modified surface of the microsensor by means of a dip-coating procedure. These microelectrodes were successfully applied for the detection of the secretion of NO from a population of human umbilical vein endothelial cells (HUVEC) into a small volume of electrolyte.