Colorectal cancer is the third most common cancer and the second most common cause of death worldwide. In the State of Palestine, it is the second most common cancer after breast cancer. Because of this widespread prevalence, detecting the causes of colon cancer has become essential. Genetic tests have become one of the most important and advanced methods for detecting cancer and to help determine the course of treatment. In this study, next-generation sequencing (NGS) technology was used to identify genetic variants that contribute to tumors in colorectal cancer.